Genetic diversity of indigenous catfish from Indonesia based on mitochondrial Cytochrome Oxidase Subunit II gene

Abstract. Budiariati V, Susmiati T, Waroh S, Putri RCA, Widayanti R. 2021. Genetic diversity of indigenous catfish from Indonesia based on mitochondrial Cytochrome Oxidase Subunit II gene. Biodiversitas 22: 593-600. Catfish is one the most demanding fish in and served a variety traditional culinary. Due to their identical morphology close relation between species order Siluriformes, it quite tricky distinguish species. This can be threat develop production since there wide this mega biodiversity country. The study aimed analyze genetic Indonesian especially those known as Baung by local people COII also determine phylogenetic relationship samples compare them with GenBank data. A total 24 used 8 different rivers 3 islands two were collected coastal areas. results showed that sequences among 691 nucleotides gene, very subtle differences originated Bojonegoro, Magelang, Baru Beach, Yogyakarta. Based amino acid sequences, 6 polymorphic sites on-site number 64, 115, 123, 129, 144, 165. encoded LLB1 LPB1 Yogyakarta, highest acids six sites. Samples river Central Java, Sumatra, Kalimantan belonged Bagridae family consist Hemibagrus sp. Mystus sp while East Java Pangasiidae family. Ariidae